Title Details

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The study developed an antibacterial periodontal irrigant using Plumeria acuminata bark extract and determined its antibacterial effect on oral pathogens. Sepcifically; it aimed to verify the secondary compounds of Plumeria acuminata bark extract through phytochemical analysis; it aimed to determine the antibacterial effect of Plumeria acuminata bark extract against Aggregatibacter actinomycetemcomitans, Fusobacterium nucleatum and Staphylococcus aureus through knowing their Zones of Inhibition; it also aimed to develop a periodontal irrigant using Plumeria acuminata bark extract. Furthermore, the significant difference in the antibacterial effect of the Plumeria acuminata bark extract against Aggregatibacter actinomycetemcomitans, Fusobacterium nucleatum and Staphylococcus aureus was also determined. Experimental treatment concentrations included 25%, 50%, and 75% of Plumeria acuminata bark extract. Chlorhexidine digluconate 20% was used as the positive control and the standard Zone of inhibition was determined by measuring the clear zones where the discs were placed. Result showed that Plumeria acuminata bark extract has an antibacterial effect, wherein 50% of the treatment concentration has the widest zone of inhibition formed in Aggregatibacter actinomycetemitans, Fusobacterium nucleatum and Staphylococcus aureus than 25% and 75% treatment concentration. The 25% treatment concentratin failed to have an effect against Aggregatibacter actinomycetemcomitans and Staphylococcus aureus; also 75% of the treatment failed to have an effect in Staphylococcus aureus and not much in Aggregatibactre actionomycetemcomitans. However, the positive control which is Chlorhexidine digluconate has the highest inhibition against all selected oral pathogens. Statistical analysis showed there was no significance compared to the significance of Chlorhexidine.

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